Phospho-Beta Catenin (Ser33) Polyclonal antibody
Phospho-Beta Catenin (Ser33) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28772-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated PC-3 cells, Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
28772-1-AP targets Phospho-Beta Catenin (Ser33) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated molecular weight | 781 aa, 86 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC058926 |
| Gene symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta Catenin/β-catenin, also known as CTNNB1, is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation (PMID: 31930681). Phosphorylated β-catenin is targeted for ubiquitination-dependent proteolysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Beta Catenin (Ser33) antibody 28772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Fusobacterium nucleatum induces oxaliplatin resistance by inhibiting ferroptosis through E-cadherin/β-catenin/GPX4 axis in colorectal cancer | ||
J Cancer MPP7 mediates EMT via Wnt/β-catenin pathway to promote polarity changes in epithelial ovarian cancer cells | ||
Exp Hematol Oncol Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway | ||
Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling |
