Prestained Protein Marker (10-180 kDa)

Molecular Weight

10-180 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00001



Product Information

The PL00001 Prestained Protein marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins. The PL00001 Prestained Protein marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4 for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa

Number of Markers

10

Size Range

10 to 180

Stain Type

3 colors: Blue, Red, Green

Publications

ApplicationTitle

Cell Rep

A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression

Authors - Ling Zhang
WB

Food Chem

Fabrication of grape seed proanthocyanidin-loaded W/O/W emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate with konjac glucomannan: Structure, stability, and in vitro digestion

Authors - Hong Zhuang

J Ethnopharmacol

Pharmacodynamics and pharmacological mechanism of Moluodan concentrated pill in the treatment of atrophic gastritis: A network pharmacological study and in vivo experiments

Authors - Ni Lou

Front Immunol

Roles of RAGE/ROCK1 Pathway in HMGB1-Induced Early Changes in Barrier Permeability of Human Pulmonary Microvascular Endothelial Cell.

Authors - Meng-Jiao Zhao

J Ethnopharmacol

The mechanisms of action of WeiChang'An Pill (WCAP) treat diarrhoea-predominant irritable bowel syndrome (IBS-D) using network pharmacology approach and in vivo studies.

Authors - Yan Chen
WB

Naunyn Schmiedebergs Arch Pharmacol

Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor.

Authors - Shangxian Luan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sara (Verified Customer) (10-06-2024)

One of my fav products. I normally use 3 ul in a well and it looks wonderful both in the gel and after transfer to the membrane. Easy to check the MW looking at the colours.

  • Applications: Western Blot
  • Cell Tissue Type: -
FH

Luidy (Verified Customer) (09-20-2024)

Excellent quality for the price

  • Applications: Western Blot,Protein purification
FH

Anastasia (Verified Customer) (08-03-2024)

Great ladder!

  • Applications: Western Blot
FH

Tatiana (Verified Customer) (06-25-2024)

Best ladder on the market - reliable results every time even with very sparing loading (2-3ul per protein gel), very competitive price. The packaging Proteintech products come in is excellent.

  • Applications: Protein purification
FH

Dipti (Verified Customer) (06-17-2024)

protein ladder is perfectly visible, 2-3 ul is enough for one gel.

  • Applications: Western Blot,Immunoprecipitation,Protein purification
  • Cell Tissue Type: thylakoid membrane
FH

Tanoy (Verified Customer) (04-08-2024)

The pre-stained protein marker yielded a nice result in my Western Blot assay.

  • Applications: Western Blot
FH

Stefanie (Verified Customer) (03-29-2024)

This protein ladder is great! The volume needed to get bright , clear bands transferred to PVDF is minimal compared to other ladders I have used. I will be transitioning to exclusively using this ladder on future blots.

  • Applications: Western Blot
FH

Clelia (Verified Customer) (03-08-2024)

Excellent protein marker. Sharpness, quality and excellent value for money.

  • Applications: Western Blot
  • Cell Tissue Type: L929
FH

Eleonora (Verified Customer) (02-29-2024)

The ladder works very good. Clear bands with bright colors. I recommend it.

  • Applications: Western Blot
FH

Emanuele (Verified Customer) (02-20-2024)

Fantastic ladder! Also with low amounts all the weights are clear!

  • Applications: Western Blot
FH

Grace (Verified Customer) (01-15-2024)

Works perfectly and no issues with transferring onto the PVDF membrane for Western Blot

  • Applications: Western Blot
FH

Tsimafei (Verified Customer) (12-11-2023)

5µl of the ladder were resolved on 4-20% polyacrylamide gel. Asymmetric position of the bands and 3 different colours make it handful to use for proteins in the range 10-180 kDa.

  • Applications: Western Blot
FH

Franziska (Verified Customer) (10-31-2023)

The Prestained Protein Marker works really good. You can see the 3 different colors in the acrilamide gel ( 12 %) and get straight bands. For protein between 10 up to 180 kDa it is a nice marker.

  • Applications: Western Blot
  • Cell Tissue Type: Hek cells
FH

Selin (Verified Customer) (08-25-2023)

Good protein ladder and good size distribution

  • Applications: Western Blot
FH

Laurens (Verified Customer) (08-01-2023)

The ladder works well and is easy to read due to triple colour banding pattern. Its very useful that the pattern is assymetric to allow for easy reading. The ladder also works for Fluorescent western blot developing. In that case you can clearly discern the banding pattern by the presence of thick bands. Personally I would however use the broad range ladder so that you can also clearly seperate larger proteins.

  • Applications: Western Blot
FH

Cristina (Verified Customer) (07-12-2023)

The ladder properly worked in an acrilamide 10% gel. The pressence of three different colors facilitate the tracking of the molecular weight. I recommend this product.

  • Applications: Western Blot
FH

Sunil (Verified Customer) (06-06-2023)

Great product

  • Applications: Western Blot
FH

Piotr (Verified Customer) (06-01-2023)

Good product. Easy to use and the marker bands are well defined. Quick delivery from Proteintech

  • Applications: Western Blot
  • Cell Tissue Type: HNSCC cancer cells
FH

Melanie (Verified Customer) (02-02-2023)

Very good marker with clean and nice bands.

  • Applications: Western Blot
  • Cell Tissue Type: HEK293T cells
FH

David (Verified Customer) (01-31-2023)

Very good protein ladder. Clear bands for our typical working range between 10 to 180 kDa

  • Applications: Western Blot
FH

Irena (Verified Customer) (11-28-2022)

Just receive the protein ladder and immediately tried with a WB! it work perfectly and it is clearly visible

  • Applications: Western Blot
FH

Christine (Verified Customer) (10-27-2022)

Very good coloured protein ladder. I loaded 1.5 ul on a SDS gel PAGE and transferred to nitrocellulose membrane and the ladder was extremely clean and visible. I will load less next time, 1 ul should be more than enough

  • Applications: Western Blot
FH

Silvia (Verified Customer) (10-18-2022)

not equal as other used but ok

  • Applications: Western Blot
FH

Agnieszka (Verified Customer) (10-14-2022)

Very versatile ladder! I do enjoy the quantity and positioning of the bands, especially under 50kDa. This ladder allowed me to assign MW to proteins which normally fall between the bands

  • Applications: Western Blot
  • Cell Tissue Type: C. elegans
FH

Filippo (Verified Customer) (09-09-2022)

Very good prestained protein marker. Clear bands and wide range.

  • Applications: Western Blot
FH

Irina (Verified Customer) (09-06-2022)

This prestained protein ladder works really good in 10-180kDa range

  • Applications: Western Blot
FH

Hasan (Verified Customer) (09-02-2022)

The samples were run on a 15% SDS-PAGE

  • Applications: Western Blot
  • Cell Tissue Type: HeLa cells
FH

FIRUZE (Verified Customer) (08-26-2022)

It works very well!

  • Applications: Western Blot
FH

Eleftheria (Verified Customer) (08-26-2022)

Accurate MW range, great staining

  • Applications: Western Blot
  • Cell Tissue Type: mouse embryonic stem cells
FH

Georgina (Verified Customer) (07-04-2022)

Great protein marker! Makes cutting membranes very easy.

  • Applications: Western Blot
  • Primary Antibody Dilution: N/A
  • Cell Tissue Type: N/A
FH

Debarpan (Verified Customer) (06-09-2022)

I found the product really promising. I compared it to PageRuler that we use traditionally and it matches the results quite perfectly.

  • Applications: Other
FH

James (Verified Customer) (05-24-2022)

Great protein ladder. Works exactly as expected :)

  • Applications: Protein purification
FH

Lucia (Verified Customer) (05-12-2022)

I used it and it worked very well, I recommend it

  • Applications: Western Blot
FH

Samuele (Verified Customer) (05-04-2022)

Reliable marker with good sharps bands and good signal even with low quantity

  • Applications: Western Blot
FH

Lisa (Verified Customer) (05-01-2022)

It works very well giving sharp and reliable bands

  • Applications: Western Blot
FH

Dalila (Verified Customer) (04-12-2022)

The marker works very good. It was easy to distinguish different bands. I used it to look at my protein of interest and house keeping, kDA bands looks very good.

  • Applications: Western Blot
  • Cell Tissue Type: Lymphocyte
FH

Irene (Verified Customer) (04-12-2022)

Perfect! It does its job. I've used it for a western blot. I recommended it.

  • Applications: Western Blot
  • Cell Tissue Type: HeLa
FH

Matteo (Verified Customer) (04-11-2022)

Used for WB, worked perfectly fine, also compared with my previous protein marker. Totally recommend

  • Applications: Western Blot
FH

Laura (Verified Customer) (04-08-2022)

Great protein ladder. Sharp bands and bright colors after transfer to nitrocellulose membrane. It comes in ready-to-use format, which is quite convenient.

  • Applications: Western Blot
FH

Brogan (Verified Customer) (04-05-2022)

very clear ladder, easy to see on PVDF membrane. Worth the price.

  • Applications: Western Blot
FH

Laura (Verified Customer) (04-01-2022)

The ladder works very well. I’ve used it to look at p53 isoforms. Good colors.

  • Applications: Western Blot
FH

AVIKA (Verified Customer) (03-19-2022)

The protein ladder worked well and the 3 different colors made the detection easier.

  • Applications: Western Blot
FH

Carlo (Verified Customer) (03-16-2022)

The protein ladder is well made and mantains color for long time

  • Applications: Western Blot
FH

camilla (Verified Customer) (03-16-2022)

i tried this as part of the phd free trial kit and i'm very satisfied of the result, i'm considering ordering this again

  • Applications: Western Blot
FH

Victoria (Verified Customer) (03-12-2022)

Clear, bright bands. Nicely colour coded for ease of use.

  • Applications: Western Blot
FH

Oliviero (Verified Customer) (03-11-2022)

Prestained proteine marker works very well in a Wb gel (1mm) with a persistent color also After washing

  • Applications: Western Blot
  • Primary Antibody Dilution: 4 uL for Gel 1 mm
FH

massimo (Verified Customer) (03-10-2022)

excellent protein markers

  • Applications: Western Blot
FH

Vincenza (Verified Customer) (03-01-2022)

The marker works really well, it is precise and bright. Excellent product. Recommended.

  • Applications: Western Blot
FH

Angelo (Verified Customer) (02-28-2022)

Great Ladder very clean and precise, I used 5uL and the color on an homemade 12% gel and the resolution it has been perfect

  • Applications: Western Blot
FH

Laura (Verified Customer) (02-25-2022)

Very defined bands

  • Applications: Western Blot
FH

Giorgia (Verified Customer) (02-25-2022)

Markers ran well and the color scale was clearly visible

  • Applications: Western Blot
FH

Cristina (Verified Customer) (02-25-2022)

Markers worked very well, with bands well defined

  • Applications: Western Blot
FH

Abigail (Verified Customer) (02-22-2022)

Ladder separated out well, colours made it really easy to read. I really like this ladder!

  • Applications: Other
FH

Alberto (Verified Customer) (02-15-2022)

First time trial. Positive impression. The ladder is just comparable to the one we normally use (other brand). It was suggested to load 3-5uL but personally I would use 10uL for a more definite ladder. No complaints.

  • Applications: Western Blot
  • Cell Tissue Type: Total protein extracts from S. cerevisiae cells
FH

Loic (Verified Customer) (02-15-2022)

Worked great for Western Blot!

  • Applications: Western Blot
FH

Alessandro (Verified Customer) (01-28-2022)

The prestained protein marker is well visibile and the color brightness is conserved in time

  • Applications: Western Blot
  • Cell Tissue Type: Melanoma
FH

Federica (Verified Customer) (01-28-2022)

prestained protein marker maintains colour brightness even after strippimng procedures.

  • Applications: Western Blot
  • Cell Tissue Type: Dendritic cells
FH

Kayleigh (Verified Customer) (01-27-2022)

good ladder, transfers well

  • Applications: Western Blot
FH

Alessandro (Verified Customer) (12-17-2021)

Worked out great, nice band separation

  • Applications: Other
FH

Luke (Verified Customer) (11-24-2021)

Ladder ran really nicely and gave clear and well defined bands on my gel

  • Applications: Western Blot
FH

Andrew (Verified Customer) (11-15-2021)

Worked well.

  • Applications: Western Blot
FH

Kai (Verified Customer) (11-05-2021)

The prestained protein ladder shows clear bands for a reasonable range of molecular weights. Including bands for smaller proteins might be useful for some cases.

  • Applications: Western Blot,Other
FH

Clara (Verified Customer) (10-15-2021)

Protein ladder used for SDS PAGE 10%, minigel, 1mm thikness. 10ul of ladder were loaded after running the gel the bands higher then the 70 kDA band disappeared

  • Applications: Western Blot
FH

Macarena (Verified Customer) (08-23-2021)

Very nice ladder. I tried using only 2uL and the bands were still clear.

  • Applications: Western Blot
FH

Maria (Verified Customer) (08-23-2021)

Cell whole lysate labelled with No-Stain (20 ug protein), VWR VI marker 9 uL, Proteintech PL00001 5 uL.

  • Applications: Western Blot
FH

Kishore (Verified Customer) (07-21-2021)

Perfect marker for protein gels, crispy bands

  • Applications: Western Blot
FH

Tatiana (Verified Customer) (07-15-2021)

Very good ladder. The bands are well separated and visible.

  • Applications: Western Blot
FH

Vasudha (Verified Customer) (07-01-2021)

The bands are nicely separated and colorful.I used 5ul but looks like 2ul would be sufficient to see the bands.

  • Applications: Western Blot
FH

Lucija (Verified Customer) (06-29-2021)

I requested a sample of Prestained protein marker 10-180 kDa) and received it in less than a week. The product is amazing, after loading only 3 uL (as I usually do), SDS-PAGE and transfer to membrane resulted in sharp and bright bands. I highly recommend this product.

  • Applications: Western Blot
FH

PRADEEP (Verified Customer) (06-27-2021)

Excellent Prestained Protein Marker

  • Applications: Western Blot
  • Cell Tissue Type: MCEC
FH

Daniel (Verified Customer) (06-11-2021)

Works exactly as you'd expect it to, no complaints. Colours are vibrant and clear. Used in SDS-PAGE. This is a higher concentration than I'm used to (2-5x more concentrated at least), you only need to use 1-3 µL in a 50µL well

  • Applications: Other
  • Cell Tissue Type: 12% 10 well 50 µL precast protein gel
FH

Yehuda (Verified Customer) (05-31-2021)

Great prestained protein ladder with clear colourful bands. Good separation in SDS-PAGE and nice transfer.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Liver
FH

Zoe (Verified Customer) (04-22-2021)

Really clear protein ladder that ran well, colours help visualisation on SDS-PAGE gel

  • Applications: Other
  • Cell Tissue Type: LOBSTR BL21 E.Coli
FH

Lucy (Verified Customer) (04-19-2021)

Nice ladder, good seperation

  • Applications: Western Blot
FH

Jessica (Verified Customer) (04-13-2021)

The protein ladder worked comparably well to the ladder that we currently use from BioRad. Unfortunately, the BioRad ladder is cheaper to buy in bulk but I wouldn't hesitate to use this one.

  • Applications: Western Blot
  • Cell Tissue Type: N/A
FH

Elizabeth (Verified Customer) (04-12-2021)

in lane on the right, tested against precision plus protein dual color standard from Bio-Rad, cat# 1610374 (left). red and green channel fluorescence in black and white included, ladder looked much better in the red channel.

  • Applications: Western Blot
FH

Ivan (Verified Customer) (04-12-2021)

Fantastic Protein Marker. Highly recommendable for any use: gels, blotting, etc. etc. etc. Clear colourful bands.

  • Applications: Western Blot
FH

T (Verified Customer) (04-08-2021)

Great protein marker, easy to use. It does the job!

  • Applications: Western Blot
FH

Brandon (Verified Customer) (03-30-2021)

The marker is easy to use and the bands are nicely separated.

  • Applications: Western Blot
FH

Ian (Verified Customer) (03-28-2021)

Product reacted well to the experiment and yielded good results.

  • Applications: Other
  • Cell Tissue Type: Cnacerous
FH

Javier (Verified Customer) (03-26-2021)

Ladder separates and aligns great my known protein.

  • Applications: Western Blot
  • Cell Tissue Type: GBM cells
FH

Elisa (Verified Customer) (03-19-2021)

I compared with other company ladder and looks pretty good, with only 4uL. Photo form a 4-20% TGX gel

  • Applications: Western Blot
FH

XIAOBING (Verified Customer) (03-02-2021)

this product works great.

  • Applications: Western Blot
FH

Julie (Verified Customer) (03-02-2021)

I like this new ladder. Just have some difficulties to observe the green ladder during migration but become better after transfer.

  • Applications: Western Blot
  • Cell Tissue Type: NRVM
FH

Pradeep (Verified Customer) (02-18-2021)

It is great.. worked well on SDS-PAGE

  • Applications: Western Blot
FH

Hala (Verified Customer) (02-08-2021)

very good working

  • Applications: Western Blot
FH

Tom (Verified Customer) (01-19-2021)

3ul of ladder loaded onto 12% acrlyamide gel. Transfer to nitrocellulose membrane and stained with ponceau to assess total protein, as seen in image.Ladder was clearly visible in both the gel and the membrane.

  • Applications: Western Blot
FH

Petra (Verified Customer) (01-12-2021)

Works well both ín SDS-polyacrylamide gel and on PVDF membranes. Load 1 ul and still clearly visible.

  • Applications: Western Blot
FH

Tom (Verified Customer) (01-08-2021)

Loaded 5uL of protein ladder into left lane on 12.5% gel, followed by transfer to nitrocellulose membrane.Ladder was clearly visible in both gel and membrane. Image captured after ponceau stain.

  • Applications: Western Blot
FH

Malak (Verified Customer) (12-28-2020)

I used this marker with Biorad precasted gels 4-15% and it separates perfectly allowing you to compare the size of your protein in a pretty accurate way. I would definitely order it for next experiments.

  • Applications: Western Blot
  • Cell Tissue Type: Protein extract
FH

Dorina (Verified Customer) (12-23-2020)

I have used this ladder a lot and it always worked reliably and consitent and I never had any issues. The bands show up strong and clearly as expected

  • Applications: Other
  • Cell Tissue Type: Primary Proximal Tubule cell lysates
FH

Kalin (Verified Customer) (12-21-2020)

It is great.

  • Applications: Western Blot
FH

Sheetal (Verified Customer) (12-14-2020)

This protein ladder was working great, it has a single reference band which avoid confusions of comparing between two or three reference bands. And it runs the same way as shown in datasheet if we run the marker in different percentages of gels.

  • Applications: Western Blot
FH

Lori (Verified Customer) (11-24-2020)

I wanted to test out 2,2,2-trichloroethanol in my resolving gel and it was good so I asked if the student would like to continue on with procedure and transfer to blot they did not have time so we did not go further than the gel electrophoresis.The ladder was bright and you could see the three distinct colors of the various bands, blue, green and red. I would definitely continue to use this product in my western blot analyses

  • Applications: Western Blot
  • Cell Tissue Type: Dorsal Root Ganglia Cells
FH

Lobarkhon (Verified Customer) (11-04-2020)

Protein Ladder works very well. I have used 4 - 5ul and both band clearly visible.With different colors easy to measure the weight of sample protein.

  • Applications: Other
  • Cell Tissue Type: Insect midgut
FH

Tamara (Verified Customer) (10-29-2020)

Good staining and good bands quality

  • Applications: Western Blot
FH

Ming Yin (Verified Customer) (10-19-2020)

The ladder worked perfectly. There were no differences in performance between this ladder and previous ladder I have used. Coloured markers were nice for images.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse hippocampus
FH

Emanuela (Verified Customer) (09-29-2020)

The ladder runs well in SDS gel loading 2,5 ul, the bands match well with the tested antibodies (actin, sigma1r, VDAC, IP3R, catalase)

  • Applications: Western Blot
  • Cell Tissue Type: Neural stem progenitor cells
FH

Satyendra (Verified Customer) (09-28-2020)

I used the protein ladder and it works fine for me

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U87-MG
FH

Bryce (Verified Customer) (09-19-2020)

This multicolored protein ladder was easy to see with only 5 uL loaded into the well.

  • Applications: Other
  • Cell Tissue Type: Urine
FH

Jie (Verified Customer) (09-01-2020)

Work well on SDS-PAGE

  • Applications: Western Blot
FH

Sarah (Verified Customer) (08-28-2020)

Good!

  • Applications: Western Blot
FH

Janardhan (Verified Customer) (08-04-2020)

I used 5ul, the bands are clearly visible and the color bands are helpful to detect the molecular weight easily

  • Applications: Western Blot
FH

Nayden (Verified Customer) (07-21-2020)

Gives clear bands on polyacrylamide gel, and on nitrocellulose membrane after standard 1h wet transfer.

  • Applications: Western Blot
FH

Onyekachim (Verified Customer) (07-21-2020)

The ladder ran as expected and was easy to detect on the gel.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse tissue
FH

Teresa (Verified Customer) (07-20-2020)

Prestained protein marker can be used 4 ul per lane. I saw bright, robust bands on my PVDF that lasted through 3 rounds of antibody incubations and ECL.

  • Applications: Western Blot
  • Cell Tissue Type: n/a
FH

pitna (Verified Customer) (07-16-2020)

1 lane : predestined protein marker from proteintech (5ul)2 lane : protein marker from lab (10ul)3, 4 lane : 5ug, 10ug Postmortem brain tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: predestined protein marker
  • Cell Tissue Type: Postmortem brain tissue
FH

Boglarka (Verified Customer) (07-14-2020)

It is easily visible and the color-coded bands are really helpful.

  • Applications: Western Blot
FH

Trisiani (Verified Customer) (07-09-2020)

The markers look nice and transferred well for Western blot.

  • Applications: Western Blot
FH

Aarthi (Verified Customer) (07-06-2020)

I needed a ladder with a 75 kDa band. The proteintech ladder worked very well for my purpose.

  • Applications: Other
FH

Rakesh (Verified Customer) (07-06-2020)

The Ladder resolves very well on 12% SDS-PAGE gel. It transfers very efficiently onto the PVDF membrane upon Western transfer. The ladder is retained on the blot even after stripping the blot. However, upon stripping the green band of the ladder disappears.

  • Applications: Western Blot
FH

deng (Verified Customer) (07-06-2020)

I recently used the protein ladder and am happy to say it works as same as product from Bio Rad dual color standards.

  • Applications: Western Blot
  • Cell Tissue Type: mouse hupothalamic N39 cells
FH

Kunny (Verified Customer) (07-06-2020)

Works great

  • Applications: Western Blot
FH

Eya (Verified Customer) (07-02-2020)

I tested this product in a wb gel at 10%, the bands were clearly visible and clear

  • Applications: Western Blot
  • Cell Tissue Type: erythroid cells
FH

Carlos (Verified Customer) (03-18-2020)

The ladder worked beautifully in our hands. The range of weights is very suitable for our studies to try fing new targets for anti-epileptic drugs

  • Applications: Western Blot
  • Cell Tissue Type: Brain tissue
FH

Phoebe (Verified Customer) (03-18-2020)

I have used this as a replacement for PageRuler Plus for a western with a large (~200kDa) protein, and it worked brilliantly.

  • Applications: Western Blot
FH

Lorraine (Verified Customer) (03-17-2020)

Worked perfectly well and will definitely consider ordering more

  • Applications: Western Blot
FH

Alejandra (Verified Customer) (03-12-2020)

Separetion of the bands was nice, th green color of the ~23kDa band was a bit less visible the other 2 colours but it was ok.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
FH

Kamila (Verified Customer) (03-11-2020)

I've used the Pritein Ladder in several Western Blot analysis and I can now say, that the bands are very clearly visible on gel and on the membrane, what makes the protein identification faster and cutting membranes easier.

  • Applications: Western Blot,
FH

Stephen (Verified Customer) (03-10-2020)

5 micro L of ladder loaded on protein 4-15% tris glycine gel . Gel wet transferred onto 0.22um nitrocellulose membrane . Membrane was stained with amido black and destained with 20% methanol 10% acetic acid Protein marker is still visible with c colours bands as well still good product

  • Applications: Western Blot,
  • Primary Antibody Dilution: 5mico L loaded
  • Cell Tissue Type: NA
FH

Petra (Verified Customer) (03-09-2020)

The ladder ran and separated well on the gel. I compared it to my normal protein ladder from ThermoFisher.I didn't image it in the end, since my actual samples didn't work that time!

  • Applications: Western Blot,
FH

Sibylle (Verified Customer) (03-09-2020)

Molecular weight ladder worked well overall, when we loaded 5ul of the pre-stained ladder. However, the high molecular bands (140-180kDa) were very faint, and then completely disappeared after the PVDF membranes were stripped to re-probe for a second marker.

  • Applications: Western Blot,
  • Cell Tissue Type: Murine brain homogenate
FH

Miray (Verified Customer) (03-05-2020)

it works well for Western blot

  • Applications: Western Blot,
FH

Anna (Verified Customer) (03-03-2020)

Very useful. I used only 3 ul and yet it looked great on the gel. I was able to follow the run and separate well the molecular weights of my interest. Having different colors makes it very easy to read.

  • Applications: Western Blot,
FH

ALBERTO (Verified Customer) (03-03-2020)

The ladder works well, detecting the bands at the expected size

  • Applications: Western Blot,
  • Cell Tissue Type: Lymphoma cell lines
FH

Sara (Verified Customer) (03-02-2020)

Nice ladder! Very easy to see, both in the gel (while running and before transfer) and in the membrane (after transfer). The transfer from the gel to membrane was almost 100%.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 3ul per lane
FH

Veronica (Verified Customer) (03-02-2020)

A good protein ladder

  • Applications: Western Blot,
  • Cell Tissue Type: retina
FH

Anton (Verified Customer) (02-26-2020)

Good ladder run on SDS-PAGE gel. Thanks!

  • Applications: Other,
FH

Laura (Verified Customer) (02-25-2020)

I liked this product compared to another one I was using before because of the 3 colors it uses.

  • Applications: Western Blot,
FH

Nicky (Verified Customer) (02-25-2020)

Excellent ladder, I liked it better than the one we currently use (SeeBlue Plus2 ladder) as the larger bands are more easily visible.

  • Applications: Western Blot,
FH

David (Verified Customer) (02-24-2020)

Ladder worked really well, very clear both on the gel and the membrane after transfer.

  • Applications: Western Blot,
FH

Deirdra (Verified Customer) (02-03-2020)

Great protein ladderClear running and the three different colours make it easy to distinguish different weights for antibody probing/ cutting membrane

  • Applications: Western Blot,
  • Cell Tissue Type: Human
FH

Serat-E (Verified Customer) (02-03-2020)

Great ladder, clear and useful. Small amount needed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000