Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11329-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11329-2-AP
WB result of RAB35 antibody (11329-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB35 transfected A431 cells.
WB analysis of MCF-7 using 11329-2-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of RAW 264.7 using 11329-2-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of HeLa using 11329-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11329-2-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11329-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11329-2-AP
IP result of anti-RAB35 (IP:11329-2-AP, 4ug; Detection:11329-2-AP 1:600) with mouse brain tissue lysate 2400 ug.
IHC staining of human breast cancer using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11329-2-AP (RAB35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11329-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11329-2-AP (RAB35 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of U-87 MG using 11329-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB35 antibody (11329-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11329-2-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (49)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, human liver tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-87 MG cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 39 publications below
IHC	See 2 publications below
IF	See 18 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

11329-2-AP targets RAB35 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1872 Product name: Recombinant human RAB35 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC015931 Sequence: MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC 相同性解析による交差性が予測される生物種
Full Name	RAB35, member RAS oncogene family
Calculated molecular weight	201 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC015931
Gene Symbol	RAB35
Gene ID (NCBI)	11021
RRID	AB_2238179
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15286
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

Protocols

Product Specific Protocols
IF protocol for RAB35 antibody 11329-2-AP	Download protocol
IHC protocol for RAB35 antibody 11329-2-AP	Download protocol
IP protocol for RAB35 antibody 11329-2-AP	Download protocol
WB protocol for RAB35 antibody 11329-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Science Identification of an oncogenic RAB protein. Authors - Douglas B Wheeler KD Validated View Article
human	WB	Cell Metab Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain. Authors - Chaitali Mukherjee View Article
human	WB	Nat Cell Biol Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. Authors - Alexander Wallroth View Article
human	WB	Nat Cell Biol An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles. Authors - James W Clancy View Article
mouse	WB,IF	Sci Adv LRRK2 mediates tubulation and vesicle sorting from lysosomes. Authors - Luis Bonet-Ponce View Article
human,mouse	WB,IHC,IF,CoIP	Nat Commun A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Authors - Corallino Salvatore KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11329-2-AP

WB analysis of A431 using 11329-2-AP

WB analysis of MCF-7 using 11329-2-AP

WB analysis of RAW 264.7 using 11329-2-AP

WB analysis of HeLa using 11329-2-AP

WB analysis of A375 using 11329-2-AP

WB analysis of human brain using 11329-2-AP

IP experiment of mouse brain using 11329-2-AP

IHC staining of human breast cancer using 11329-2-AP

IHC staining of human breast cancer using 11329-2-AP

IHC staining of human liver using 11329-2-AP

IHC staining of human liver using 11329-2-AP

IHC staining of human brain using 11329-2-AP

IHC staining of human brain using 11329-2-AP

IF Staining of U-87 MG using 11329-2-AP

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of an oncogenic RAB protein.

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.