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- KD/KO Validated
RAD52 Polyclonal antibody
RAD52 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 28045-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HL-60 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
Product Information
28045-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAD52 fusion protein Ag27619 相同性解析による交差性が予測される生物種 |
| Full Name | RAD52 homolog (S. cerevisiae) |
| Calculated molecular weight | 46 aa |
| Observed molecular weight | 46-50 kDa |
| GenBank accession number | NM_001297419 |
| Gene symbol | RAD52 |
| Gene ID (NCBI) | 5893 |
| RRID | AB_2881046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD52 antibody 28045-1-AP | Download protocol |
| IP protocol for RAD52 antibody 28045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Int J Biochem Cell Biol Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors. | ||
Cell Death Dis SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.
| ||
Nat Commun Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (10-15-2024) | I detected the expected band for RAD52 and 48 kDa in WT cells, but not RAD52 KO cells, proving that the antibody is indeed recognizing RAD52.
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