Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of MCF-7 using 10806-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10806-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10806-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10806-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 10806-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10806-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human skin cancer using 10806-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10806-1-AP (RFC4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 10806-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10806-1-AP (RFC4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 10806-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of U2OS using 10806-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10806-1-AP
Immunofluorescent analysis of HepG2 cells, using RFC4 antibody 10806-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HepG2 using 10806-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RFC4 Polyclonal antibody (10806-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RFC4 Polyclonal antibody

RFC4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10806-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

RFC37, RF-C 37 kDa subunit, A1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, LNCaP cells, Raji cells
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells, HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

10806-1-AP targets RFC4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RFC4 fusion protein Ag1234 相同性解析による交差性が予測される生物種
Full Name	replication factor C (activator 1) 4, 37kDa
Calculated molecular weight	37 kDa
Observed molecular weight	37-40 kDa
GenBank accession number	BC017452
Gene Symbol	RFC4
Gene ID (NCBI)	5984
RRID	AB_2178477
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35249
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RFC4 antibody 10806-1-AP	Download protocol
IHC protocol for RFC4 antibody 10806-1-AP	Download protocol
IF protocol for RFC4 antibody 10806-1-AP	Download protocol
FC protocol for RFC4 antibody 10806-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Aging (Albany NY) The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis. Authors - Jing Wang View Article
mouse	WB	Cell Rep Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. Authors - Stephen Gray View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 10806-1-AP

WB analysis of Raji using 10806-1-AP

WB analysis of MCF-7 using 10806-1-AP

WB analysis of Raji using 10806-1-AP

WB analysis of LNCaP using 10806-1-AP

WB analysis of Raji using 10806-1-AP

IHC staining of human skin cancer using 10806-1-AP

IHC staining of human skin cancer using 10806-1-AP

IF Staining of U2OS using 10806-1-AP

IF Staining of U2OS using 10806-1-AP

IF Staining of HepG2 using 10806-1-AP

FC experiment of HepG2 using 10806-1-AP

RFC4 Polyclonal antibody

RFC4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.