Validation Data Gallery

Filter: AllWB

WB analysis using 12448-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12448-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis using 12448-1-AP
K-562 cell lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12448-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12448-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

RPA1 Polyclonal antibody

RPA1 Polyclonal Antibody for WB, ELISA

Cat No. 12448-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HEK-293 cells, K-562 cells, Y79 cells, A431 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

12448-1-AP targets RPA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPA1 fusion protein Ag3067 相同性解析による交差性が予測される生物種
Full Name	replication protein A1, 70kDa
Calculated molecular weight	616 aa, 68 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC018126
Gene Symbol	RPA1
Gene ID (NCBI)	6117
RRID	AB_2180495
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27694
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Replication protein A 1 (RPA1) is a subunit of a stable heterotrimer replication protein A (RPA), which is conserved in almost all eukaryotic species (PMID: 27151292). Usually, RPA1, RPA2 and RPA3 function as a whole to participate in DNA replication, telomere maintenance, DNA recombination, DNA repair, and activation of DNA damage checkpoints. In the cell, RPA has a higher affinity to bind single strand DNA and then to form a fork protection complex with other components such as PTEN and OTUB1, thus maintains genome stability during replication(PMID: 26403191; PMID: 18469000).

Protocols

Product Specific Protocols
WB protocol for RPA1 antibody 12448-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Oncol PMID: 31249811 Authors - Ma-Sha Huang View Article

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