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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPA4 Polyclonal antibody

RPA4 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 18144-1-AP

Synonyms

HSU24186, Replication factor A protein 4, replication protein A4, 34kDa, RF A protein 4, RP A p30, RPA4

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 18144-1-AP

    WB analysis of HeLa using 18144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 18144-1-AP

    WB analysis of mouse spleen using 18144-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18144-1-AP (RPA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18144-1-AP

    IP experiment of HeLa using 18144-1-AP

    IP result of anti-RPA4 (IP:18144-1-AP, 4ug; Detection:18144-1-AP 1:300) with HeLa cells lysate 3400ug.

Tested Applications

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

18144-1-AP targets RPA4 in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA4 fusion protein Ag12800 相同性解析による交差性が予測される生物種
Full Name replication protein A4, 34kDa
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC069791
Gene symbol RPA4
Gene ID (NCBI) 29935
RRIDAB_2180970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPA4, also named as RP-A p30, is a 261 amino acid protein, which belongs to the replication factor A protein 2 family. RPA4 is preferentially expressed in placental and colon mucosa. RPA4 as part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. This protein, Rpa4, has 47% identity with Rpa2, the 34-kDa subunit of RPA. (PMID: 7760808)

Protocols

Product Specific Protocols
WB protocol for RPA4 antibody 18144-1-APDownload protocol
IP protocol for RPA4 antibody 18144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols