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  • KD/KO Validated

RPL24 Polyclonal antibody

RPL24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 17082-1-AP

Synonyms

ribosomal protein L24, Large ribosomal subunit protein eL24, 60S ribosomal protein L30, 60S ribosomal protein L24


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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman placenta tissue, human kidney tissue, human liver tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17082-1-AP targets RPL24 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL24 fusion protein Ag7085 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L24
Calculated molecular weight 18 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC000690
Gene symbol RPL24
Gene ID (NCBI) 6152
RRIDAB_2181728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]

Protocols

Product Specific Protocols
WB protocol for RPL24 antibody 17082-1-APDownload protocol
IHC protocol for RPL24 antibody 17082-1-APDownload protocol
IF protocol for RPL24 antibody 17082-1-APDownload protocol
IP protocol for RPL24 antibody 17082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.

Authors - Sridevi Challa
  • KD Validated
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
IF

Neuron

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

Authors - Roberta Cagnetta
WB

Dev Cell

Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions

Authors - Max Koppers
humanIHC, WB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
WB,IP

Cell Mol Life Sci

Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner

Authors - Yonat Tzur

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Erica (Verified Customer) (05-15-2019)

The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: Mammalian cells