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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RUVBL2 Monoclonal an (CL488-67851)

    IF Staining of HepG2 using CL488-67851

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RUVBL2 antibody (CL488-67851, Clone: 1A1A4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HL-60 cells using CoraLite® Plus 488-conjugated RUVBL2 Monoclonal an (CL488-67851)

    FC experiment of HL-60 using CL488-67851

    1X10^6 HL-60 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RUVBL2 (CL488-67851, Clone:1A1A4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated RUVBL2 Monoclonal antibody

RUVBL2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67851
Clone No.1A1A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

RUVB2, ECP-51, EC:3.6.4.12, CGI-46, 48 kDa TBP-interacting protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHL-60 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67851 targets RUVBL2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RUVBL2 fusion protein Ag0253 相同性解析による交差性が予測される生物種
Full Name RuvB-like 2 (E. coli)
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC000428
Gene Symbol RUVBL2
Gene ID (NCBI) 10856
RRIDAB_2934575
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y230
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RUVBL2 antibody CL488-67851Download protocol
Standard Protocols
Click here to view our Standard Protocols