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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using SARS-COV-2 NSP5 Polyclonal antibody (29286-1-AP)

    IF Staining of HEK-293T using 29286-1-AP

    Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

  • ELISA experiment using SARS-COV-2 NSP5 Polyclonal antibody (29286-1-AP)

    ELISA experiment using 29286-1-AP

    SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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SARS-COV-2 NSP5 Polyclonal antibody

SARS-COV-2 NSP5 Polyclonal Antibody for IF, ELISA
Cat No. 29286-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

virus and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

SARS-COV-2 NSP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHEK-293T cells
Positive ELISA detected inN/A

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

29286-1-AP targets SARS-COV-2 NSP5 in WB, IF/ICC, ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-COV-2 NSP5 fusion protein Ag30934 相同性解析による交差性が予測される生物種
Full Name ORF1ab
GenBank accession numberYP_009742612
Gene Symbol SARS-COV-2 NSP8
Gene ID (NCBI) 43740578
RRIDAB_2918269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12

Authors - Jinping Yang
View Article
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article