SIRT5 Monoclonal antibody
SIRT5 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1F2B2
Cat no : 67257-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, L02 cells, LNCaP cells, K-562 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
67257-1-Ig targets SIRT5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SIRT5 fusion protein Ag7743 相同性解析による交差性が予測される生物種 |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC000126 |
| Gene symbol | SIRT5 |
| Gene ID (NCBI) | 23408 |
| RRID | AB_2882530 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT5 antibody 67257-1-Ig | Download protocol |
| IHC protocol for SIRT5 antibody 67257-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. | ||
Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma |
