Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
55041-1-AP targets SOS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | son of sevenless homolog 1 (Drosophila) |
| Calculated molecular weight | 152 kDa |
| Observed molecular weight | 152 kDa |
| GenBank accession number | NM_005633 |
| Gene Symbol | SOS1 |
| Gene ID (NCBI) | 6654 |
| RRID | AB_10804659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q07889 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IHC protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IF protocol for SOS1 antibody 55041-1-AP | Download protocol |
| IP protocol for SOS1 antibody 55041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cells Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer. | ||
Pathol Res Pract KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway | ||
Cancers (Basel) Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling | ||
Adv Sci (Weinh) Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer | ||
Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis |