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TMED9 Polyclonal antibody

Name: TMED9 Polyclonal antibody
Brand: Proteintech
SKU: 21620-1-AP
Rating: 4.00 (1 reviews)

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 21620-1-AP

Synonyms

p25, p24alpha2, p24 family protein alpha-2, GP25L2, GMP25

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 21620-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 21620-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 21620-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 21620-1-AP
pig liver tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21620-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21620-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 21620-1-AP
IP result of anti-TMED9 (IP:21620-1-AP, 4ug; Detection:21620-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 21620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 21620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 21620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21620-1-AP (TMED9 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 21620-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HEK-293 using 21620-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 21620-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

21620-1-AP targets TMED9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMED9 fusion protein Ag13813 相同性解析による交差性が予測される生物種
Full Name	transmembrane emp24 protein transport domain containing 9
Calculated molecular weight	235 aa, 27 kDa
Observed molecular weight	24-27 kDa
GenBank accession number	BC001123
Gene symbol	TMED9
Gene ID (NCBI)	54732
RRID	AB_10858623
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).

Protocols

Product Specific Protocols
WB protocol for TMED9 antibody 21620-1-AP	Download protocol
IHC protocol for TMED9 antibody 21620-1-AP	Download protocol
IF protocol for TMED9 antibody 21620-1-AP	Download protocol
IP protocol for TMED9 antibody 21620-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. Authors - Moran Dvela-Levitt View Article
human	WB	Cell A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion. Authors - Min Zhang KO Validated View Article
human	WB,CoIP	Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis. Authors - Shulin Li KD Validated View Article
human	WB	BMC Biol ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier. Authors - Xihua Yue View Article
	WB	Mol Biol Cell The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Authors - Benjamin S Roberts View Article
human	WB,IHC	Bioengineered High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma. Authors - Gaoda Ju KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Giulia (Verified Customer) (02-29-2024)

Specific antibody that works well in Western blot, both for cells and tissue lysates.

Applications: Western Blot, Immunofluorescence

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMED9 Polyclonal antibody

TMED9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 21620-1-AP

WB analysis using 21620-1-AP

WB analysis of mouse pancreas using 21620-1-AP

WB analysis of pig liver using 21620-1-AP

WB analysis of mouse brain using 21620-1-AP

WB analysis of mouse lung using 21620-1-AP

IP experiment of mouse liver using 21620-1-AP

IHC staining of human liver using 21620-1-AP

IHC staining of human liver using 21620-1-AP

IHC staining of human liver using 21620-1-AP

IF Staining of HepG2 using 21620-1-AP

IF Staining of HEK-293 using 21620-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes

High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.

Reviews