Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 14437-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 14437-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 14437-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 14437-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14437-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 14437-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 14437-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

TMPRSS2 Polyclonal antibody

TMPRSS2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 14437-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain, PRSS10, PP9284, EC:3.4.21.122

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, Caco-2 cells, T-47D cells
Positive IHC detected in	human colon cancer tissue, human prostate cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 26 publications below
IHC	See 4 publications below
IF	See 10 publications below

Product Information

14437-1-AP targets TMPRSS2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMPRSS2 fusion protein Ag5824 相同性解析による交差性が予測される生物種
Full Name	transmembrane protease, serine 2
Calculated molecular weight	54 kDa
Observed molecular weight	70, 54, 31 kDa
GenBank accession number	BC051839
Gene Symbol	TMPRSS2
Gene ID (NCBI)	7113
RRID	AB_2878058
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15393
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

Protocols

Product Specific Protocols
WB protocol for TMPRSS2 antibody 14437-1-AP	Download protocol
IHC protocol for TMPRSS2 antibody 14437-1-AP	Download protocol
IF protocol for TMPRSS2 antibody 14437-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Microbiol SARS-CoV-2 Omicron is an immune escape variant with an altered cell entry pathway. Authors - Brian J Willett View Article
human	WB	Allergy Distinct expression of SARS-CoV-2 receptor ACE2 correlates with endotypes of chronic rhinosinusitis with nasal polyps. Authors - Ming Wang View Article
human	WB	Eur Respir J Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells. Authors - Kelly S Schweitzer View Article
human	WB	Theranostics Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism. Authors - Qian Zhang View Article
human	WB	Mol Ther Nucleic Acids The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2. Authors - Guifang Du View Article
human	IF	PLoS Pathog Infection of human Nasal Epithelial Cells with SARS-CoV-2 and a 382-nt deletion isolate lacking ORF8 reveals similar viral kinetics and host transcriptional profiles. Authors - Akshamal M Gamage View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14437-1-AP

WB analysis using 14437-1-AP

WB analysis of COLO 320 using 14437-1-AP

WB analysis of COLO 320 using 14437-1-AP

IHC staining of human colon cancer using 14437-1-AP

IHC staining of human colon cancer using 14437-1-AP

IHC staining of mouse kidney using 14437-1-AP

IHC staining of mouse kidney using 14437-1-AP

IHC staining of human prostate cancer using 14437-1-AP

IHC staining of human prostate cancer using 14437-1-AP

IF Staining of human tonsillitis using 14437-1-AP

IF Staining of human tonsillitis using 14437-1-AP

TMPRSS2 Polyclonal antibody

TMPRSS2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SARS-CoV-2 Omicron is an immune escape variant with an altered cell entry pathway.

Distinct expression of SARS-CoV-2 receptor ACE2 correlates with endotypes of chronic rhinosinusitis with nasal polyps.

Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.

Infection of human Nasal Epithelial Cells with SARS-CoV-2 and a 382-nt deletion isolate lacking ORF8 reveals similar viral kinetics and host transcriptional profiles.