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TMPRSS2 抗体 13 文献

Rabbit Polyclonal| カタログ番号: 14437-1-AP

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適用アプリケーション

アプリケーション:
WB, IHC, IF, ELISA
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Images See all

TMPRSS2 Antibody WB COLO 320 cells 14437-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMPRSS2 Antibody IF human tonsillitis tissue 14437-1-AP

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TMPRSS2 Antibody IF human tonsillitis tissue 14437-1-AP

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TMPRSS2 Antibody IHC human colon cancer tissue 14437-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMPRSS2 Antibody IHC human colon cancer tissue 14437-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

種特異性:
human, mouse, rat
WB ポジティブ:
COLO 320 cells, Caco-2 cells
IHC ポジティブ:
human colon cancer tissue, human prostate cancer tissue
IF ポジティブ:
human tonsillitis tissue
推奨希釈倍率:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
TMPRSS2 fusion protein Ag5824
正式名:
transmembrane protease, serine 2
計算分子量:
54 kDa
検出分子量:
70, 54, 31 kDa
GenBank accession number:
BC051839
Gene ID (NCBI):
7113
遺伝子シンボル:
TMPRSS2
別名:
PP9284, PRSS10, Serine protease 10, TMPRSS2

背景説明

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 as a host cell factor that is critical for spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved in to some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).


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