Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 15202-1-AP
WB result of KAP1 antibody (15202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HeLa cells.
WB analysis of HeLa using 15202-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15202-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15202-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15202-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15202-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15202-1-AP
IP result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human colon using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15202-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 15202-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 15202-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KAP1 (15202-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15202-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA

Synonyms

TRIM28, KRAB interacting protein 1, KRAB associated protein 1, KAP-1, KAP 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (40)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 36 publications below
IHC	See 7 publications below
IF	See 5 publications below
IP	See 5 publications below
CoIP	See 2 publications below
ChIP	See 3 publications below

Product Information

15202-1-AP targets KAP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KAP1 fusion protein Ag7350 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 28
Calculated molecular weight	89 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC004978
Gene Symbol	KAP1
Gene ID (NCBI)	10155
RRID	AB_2209890
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13263
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

Protocols

Product Specific Protocols
WB protocol for KAP1 antibody 15202-1-AP	Download protocol
IHC protocol for KAP1 antibody 15202-1-AP	Download protocol
IF protocol for KAP1 antibody 15202-1-AP	Download protocol
IP protocol for KAP1 antibody 15202-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers Authors - Zhiwen Ding View Article
mouse	WB, IHC	Hepatology Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma. Authors - Bojkowska Karolina K KO Validated View Article
mouse	WB,IP	J Exp Med ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons. Authors - Longfei Ma View Article
mouse	WB	Nucleic Acids Res Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency. Authors - Weihua Qin KO Validated View Article
human	chIP	Proc Natl Acad Sci U S A TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks. Authors - Min Li View Article
mouse	WB,ChIP	Cell Rep POGZ suppresses 2C transcriptional program and retrotransposable elements Authors - Xiaoyun Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15202-1-AP

WB analysis of HeLa using 15202-1-AP

WB analysis using 15202-1-AP

WB analysis of rat spleen using 15202-1-AP

WB analysis of mouse testis using 15202-1-AP

WB analysis of HeLa using 15202-1-AP

IP experiment of HeLa using 15202-1-AP

IHC staining of human colon using 15202-1-AP

IHC staining of human lung cancer using 15202-1-AP

IHC staining of human lung cancer using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

IF Staining of HeLa using 15202-1-AP

FC experiment of HeLa using 15202-1-AP

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

POGZ suppresses 2C transcriptional program and retrotransposable elements