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UHRF1 Polyclonal antibody

Name: UHRF1 Polyclonal antibody
Brand: Proteintech
SKU: 21402-1-AP

UHRF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21402-1-AP

Synonyms

hNP95, HuNp95, ICBP90, Nuclear protein 95, RING finger protein 106, RNF106, Transcription factor ICBP90, UHRF1

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21402-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21402-1-AP
IP result of anti-UHRF1 (IP:21402-1-AP, 4ug; Detection:21402-1-AP 1:1000) with HeLa cells lysate 3200ug.
IF Staining of HepG2 using 21402-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (21402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 21402-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 19 publications below
IHC	See 4 publications below
IF	See 3 publications below
CoIP	See 3 publications below
RIP	See 1 publications below

Product Information

21402-1-AP targets UHRF1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UHRF1 fusion protein Ag16111 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-like with PHD and ring finger domains 1
Calculated molecular weight	806 aa, 91 kDa
Observed molecular weight	91 kDa
GenBank accession number	BC113875
Gene symbol	UHRF1
Gene ID (NCBI)	29128
RRID	AB_10860902
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

Protocols

Product Specific Protocols
WB protocol for UHRF1 antibody 21402-1-AP	Download protocol
IF protocol for UHRF1 antibody 21402-1-AP	Download protocol
IP protocol for UHRF1 antibody 21402-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1. Authors - Huifang Zhang View Article
human	WB	Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Authors - Qi Zhang View Article
human	WB	Cell Discov USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment. Authors - Jialun Li KD Validated View Article
human	WB	Int J Mol Sci Identification of Overexpressed Genes in Malignant Pleural Mesothelioma. Authors - Federica Morani View Article
mouse	CoIP,RIP	Stem Cell Reports UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells. Authors - Shumin Zhou View Article
	WB,IHC	Inflammation Upregulation of UHRF1 Promotes PINK1-mediated Mitophagy to Alleviates Ferroptosis in Diabetic Nephropathy Authors - Hongfei Ji View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UHRF1 Polyclonal antibody

UHRF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 21402-1-AP

WB analysis of MCF-7 using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of MCF-7 using 21402-1-AP

WB analysis of MCF-7 using 21402-1-AP

WB analysis of MCF-7 using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of HeLa using 21402-1-AP

WB analysis of MCF-7 using 21402-1-AP

IP experiment of HeLa using 21402-1-AP

IF Staining of HepG2 using 21402-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1.

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells.

Upregulation of UHRF1 Promotes PINK1-mediated Mitophagy to Alleviates Ferroptosis in Diabetic Nephropathy