Featured Product
KD/KO Validated

USP28 Polyclonal antibody

Name: USP28 Polyclonal antibody
Brand: Proteintech
SKU: 17707-1-AP
Rating: 5.00 (1 reviews)

USP28 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 17707-1-AP

Synonyms

Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of HeLa using 17707-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 17707-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 17707-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of HeLa using 17707-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17707-1-AP (USP28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17707-1-AP
IP result of anti-USP28 (IP:17707-1-AP, 4ug; Detection:17707-1-AP 1:600) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 17707-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17707-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17707-1-AP (USP28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 17707-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, mouse heart tissue, MDA-MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 18 publications below
IHC	See 5 publications below
IF	See 7 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

17707-1-AP targets USP28 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP28 fusion protein Ag12003 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 28
Calculated molecular weight	583aa,66 kDa; 1077aa,120 kDa
Observed molecular weight	135 kDa
GenBank accession number	BC065928
Gene symbol	USP28
Gene ID (NCBI)	57646
RRID	AB_2272676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

Protocols

Product Specific Protocols
WB protocol for USP28 antibody 17707-1-AP	Download protocol
IHC protocol for USP28 antibody 17707-1-AP	Download protocol
IP protocol for USP28 antibody 17707-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	J Clin Invest The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer. Authors - Markus E Diefenbacher KD Validated View Article
human	WB	Cell Death Differ Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc. Authors - Yunhua Peng KO Validated View Article
human	IF	EMBO J ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification. Authors - Lauren T Evans View Article
human	IHC	Cell Rep The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells. Authors - Wu Yadi Y View Article
human	IF	J Cell Biol A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Authors - Bramwell G Lambrus View Article
human,mouse	WB,IHC	J Biol Chem The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells Authors - Junjun Li KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Nikhil (Verified Customer) (11-10-2019)

Detect endogenous USP28 in A549 cell line

Applications: Western Blot,
Primary Antibody Dilution: 1:1500
Cell Tissue Type: A549

USP28 Antibody Western Blot, validation (1:1500 dilution) in A549 (Cat no:17707-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP28 Polyclonal antibody

USP28 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 17707-1-AP

WB analysis using 17707-1-AP

WB analysis of mouse heart using 17707-1-AP

WB analysis of HeLa using 17707-1-AP

IP experiment of HeLa using 17707-1-AP

IHC staining of human breast cancer using 17707-1-AP

IHC staining of human breast cancer using 17707-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.

Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.

ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

The Deubiquitinase USP28 Stabilizes LSD1 and Confers Stem-Cell-like Traits to Breast Cancer Cells.

A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis.

The Deubiquitinase USP28 Maintains the Expression of the Transcription Factor MYCN and Is Essential in Neuroblastoma Cells

Reviews