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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN alpha 2A protein

Activity

Typically ≤ 0.06 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1066

Synonyms

IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Validation Data Gallery

  • Purity of recombinant human IFN alpha 2A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IFN alpha 2A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IFN alpha 2A (HZ-1066) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN alpha 2A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.

    Recombinant human IFN alpha 2A (HZ-1066) dose-dependently inhibits growth of the TF-1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IFN alpha 2A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.

Technical Specifications

GeneID 3440
Species Human
Expression HEK293
Activity Typically ≤ 0.06 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01563
Molecular Mass 18 to 22 kDa reduced, 14 to 19 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761).

Synonyms

IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Publications

SpeciesTitle
human

Ann Rheum Dis

Parsing multiomics landscape of activated synovial fibroblasts highlights drug targets linked to genetic risk of rheumatoid arthritis.

Authors - Haruka Tsuchiya
View Article

J Am Chem Soc

Using an Old Drug to Target a New Drug Site: Application of Disulfiram to Target the Zn-Site in HCV NS5A Protein.

Authors - Yu-Ming Lee
View Article
human

Int J Mol Sci

Interferon-Induced Transmembrane Protein 1 (IFITM1) Promotes Distant Metastasis of Small Cell Lung Cancer.

Authors - Shuichi Sakamoto
View Article
human

Cell Mol Immunol

BCR-ABL-specific CD4+ T-helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells.

Authors - NorihiroUeda
View Article
human

ACS Infect Dis

The Nonstructural Protein of Guertu Virus Disrupts Host Defenses by Blocking Antiviral Interferon Induction and Action.

Authors - Yuan-Qin Min
View Article
human

Cancer Sci

Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells.

Authors - Yue Huang
View Article