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  • KD/KO Validated

UBE2B Polyclonal antibody

UBE2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10733-1-AP

Synonyms

E2 17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin protein ligase B



Tested Applications

Positive WB detected inL02 cells, Transfected HEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10733-1-AP targets UBE2B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2B fusion protein Ag1147
Full Name ubiquitin-conjugating enzyme E2B (RAD6 homolog)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC005979
Gene Symbol UBE2B
Gene ID (NCBI) 7320
RRIDAB_2212174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2B (Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway (PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modifications occur (PMID:10373550).

Protocols

Product Specific Protocols
WB protocol for UBE2B antibody 10733-1-APDownload protocol
IHC protocol for UBE2B antibody 10733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Biol Reprod

Identification of Ube2b as a Novel Target of Androgen Receptor in Mouse Sertoli Cells.

Authors - Mou Lisha L
  • KD Validated

Int J Mol Med

Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.

Authors - Yu-Chi Li
humanWB,IHC,IF

Bioengineered

UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.

Authors - Xi Zeng
  • KD Validated
humanWB

Nat Commun

Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes

Authors - Wenjing Yan