Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse testis using 16939-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 16939-1-AP
WB result of GALNT14 antibody (16939-1-AP; 1:1200; room temperature for 1.5 hours) with wild-type and GALNT14 knockout A549 cells.
WB analysis of mouse lung using 16939-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16939-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 16939-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 Antibody) at dilution of 1:400 incubated at 4 degree celsius over night.
IHC staining of human kidney using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human spleen using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 16939-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

GALNT14 Polyclonal antibody

GALNT14 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16939-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GalNac T10, GalNac T14, GALNT14, GALNT15, pp GaNTase 14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse testis tissue, mouse kidney tissue, mouse lung tissue, rat testis tissue
Positive IHC detected in	human kidney tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 3 publications below

Product Information

16939-1-AP targets GALNT14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10470 Product name: Recombinant human GALNT14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 203-552 aa of BC010659 Sequence: EVNRDWLQPLLHRVKEDYTRVVCPVIDIINLDTFTYIESASELRGGFDWSLHFQWEQLSPEQKARRLDPTEPIRTPIIAGGLFVIDKAWFDYLGKYDMDMDIWGGENFEISFRVWMCGGSLEIVPCSRVGHVFRKKHPYVFPDGNANTYIKNTKRTAEVWMDEYKQYYYAARPFALERPFGNVESRLDLRKNLRCQSFKWYLENIYPELSIPKESSIQKGNIRQRQKCLESQRQNNQETPNLKLSPCAKVKGEDAKSQVWAFTYTQQILQEELCLSVITLFPGAPVVLVLCKNGDDRQQWTKTGSHIEHIASHLCLDTDMFGDGTENGKEIVVNPCESSLMSQHWDMVSS 相同性解析による交差性が予測される生物種
Full Name	UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14)
Calculated molecular weight	552 aa, 64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC010659
Gene Symbol	GALNT14
Gene ID (NCBI)	79623
RRID	AB_2232200
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96FL9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for GALNT14 antibody 16939-1-AP	Download protocol
WB protocol for GALNT14 antibody 16939-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma. Authors - Ok-Seon Kwon KD Validated View Article
		Mol Cancer Res BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14. Authors - Joanna C Hillman View Article
human	WB,IHC	Cell Signal GALNT14-mediated O-glycosylation drives lung adenocarcinoma progression by reducing endogenous reactive oxygen species generation Authors - Bingbing Tang View Article
human	WB,IHC	Cancer Biomark Rs9679162 genotype predicts prognosis of real-world advanced hepatocellular carcinoma treated by sorafenib Authors - Chih-Lang Lin View Article
human	IHC	Front Immunol Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma Authors - Jintao Wu View Article
human	WB	Front Immunol Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma Authors - Zhongyue Liu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse testis using 16939-1-AP

WB analysis of A549 using 16939-1-AP

WB analysis of mouse lung using 16939-1-AP

WB analysis of mouse kidney using 16939-1-AP

WB analysis of rat testis using 16939-1-AP

IHC staining of human kidney using 16939-1-AP

IHC staining of human kidney using 16939-1-AP

IHC staining of human testis using 16939-1-AP

IHC staining of human testis using 16939-1-AP

IHC staining of human skin using 16939-1-AP

IHC staining of human skin using 16939-1-AP

IHC staining of human liver using 16939-1-AP

IHC staining of human liver using 16939-1-AP

IHC staining of human spleen using 16939-1-AP

IHC staining of human spleen using 16939-1-AP

IHC staining of human ovary using 16939-1-AP

IHC staining of human ovary using 16939-1-AP

IHC staining of human heart using 16939-1-AP

IHC staining of human heart using 16939-1-AP

GALNT14 Polyclonal antibody

GALNT14 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma.

BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14.

GALNT14-mediated O-glycosylation drives lung adenocarcinoma progression by reducing endogenous reactive oxygen species generation

Rs9679162 genotype predicts prognosis of real-world advanced hepatocellular carcinoma treated by sorafenib

Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma

Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma