Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, NCI-H1299 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24694-1-AP targets KNTC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20268 Product name: Recombinant human KNTC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1859-2209 aa of BC150278 Sequence: DYSSRMLFVFATSTTTTLGMHQLTFAHRTRALQCLFYLADKETIESLFKKPIEEVKSYLRCITFLASFETLNIPITYELFCSSPKEGMIKGLWKNHSHESMAVRLVTELCLEYKIYDLQLWNGLLQKLLGFNMIPYLRKVLKAISSIHSLWQVPYFSKAWQRVIQIPLLSASCPLSPDQLSDCSESLIAVLECPVSGDLDLIGVARQYIQLELPAFALACLMLMPHSEKRHQQIKNFLGSCDPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIINKKEFGILAKTKYFQMLKMHAMNTNNITELVNYLANDLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMFLSGLS 相同性解析による交差性が予測される生物種 |
| Full Name | kinetochore associated 1 |
| Calculated molecular weight | 251 kDa |
| Observed molecular weight | 210-230 kDa |
| GenBank accession number | BC150278 |
| Gene Symbol | KNTC1 |
| Gene ID (NCBI) | 9735 |
| RRID | AB_3669445 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KNTC1 is a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Northern blot analysis revealed low-to-moderate expression in all tissues tested, with highest expression in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KNTC1 antibody 24694-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |