F4/80 Polyclonal antibody
F4/80 Polyclonal Antibody for IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse, Rat and More (4)
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
157
Cat no : 28463-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse spleen tissue, mouse liver tissue, RAW 264.7 cells |
Positive FC detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:4000-1:12000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 12 publications below |
IHC | See 83 publications below |
IF | See 60 publications below |
FC | See 8 publications below |
Product Information
28463-1-AP targets F4/80 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.
Tested Reactivity | Mouse, Rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | F4/80 fusion protein Ag28252 |
Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
Calculated Molecular Weight | 102 kDa |
GenBank Accession Number | NM_010130 |
Gene Symbol | Emr1 |
Gene ID (NCBI) | 13733 |
RRID | AB_2881149 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
Product Specific Protocols | |
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IHC protocol for F4/80 antibody 28463-1-AP | Download protocol |
IF protocol for F4/80 antibody 28463-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia. | ||
Biomaterials A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis. | ||
Aging Dis P-Glycoprotein Aggravates Blood Brain Barrier Dysfunction in Experimental Ischemic Stroke by Inhibiting Endothelial Autophagy | ||
Autophagy Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. | ||
Acta Biomater An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kannan (Verified Customer) (06-13-2023) | AR using EDTApH9. overnight incubation of primary antibody at 4c.
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