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IDH1 Monoclonal antibody, PBS Only

IDH1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6A2

Cat no : 66197-1-PBS

Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of DU 145 using 66197-1-Ig (same clone as 66197-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of MCF-7 using 66197-1-Ig (same clone as 66197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IDH1 Monoclonal antibody (66197-1-Ig)

    WB analysis of HeLa using 66197-1-Ig (same clone as 66197-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human pancreas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IDH1 Monoclonal antibody (66197-1-Ig)

    IHC staining of human gliomas using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH1 Monoclonal antibody (66197-1-Ig)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66197-1-PBS targets IDH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH1 fusion protein Ag19293
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).