• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse IgG2b isotype control Monoclonal antibody, PBS Only

Mouse IgG2b isotype control Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-PBS

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

    Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig)

    FC experiment of Jurkat using 66360-3-Ig (same clone as 66360-3-PBS)

    1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66360-3-PBS targets Mouse IgG2b isotype control in WB, FC, IHC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2b isotype control
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).