×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MOLT-4 cells using CD8a Monoclonal antibody (66868-1-Ig)

    WB analysis of MOLT-4 using 66868-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using CD8a Monoclonal antibody (66868-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66868-1-Ig

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66868-1-Ig (CD8a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human spleen using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lymphoma using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human colon using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human tonsillitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66868-1-Ig (CD8 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human pancreas cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human pancreas cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human appendicitis using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:4000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lung cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human breast cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human breast cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lung cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lung cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human pancreas cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human pancreas cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human colon cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human colon cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human colon cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human colon cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human breast cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human breast cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IHC staining of human lung cancer using 66868-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66868-1-Ig (CD8a antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IF Staining of human tonsillitis using 66868-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD8a Monoclonal antibody (66868-1-Ig)

    IF Staining of human tonsillitis using 66868-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD8a antibody (66868-1-Ig, Clone: 1G2B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD8a Monoclonal antibody

CD8a Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66868-1-Ig
Clone No.1G2B10
Publications(334)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD8, 1G2B10, CD8a molecule, Leu2, MAL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, human breast cancer tissue, human colon cancer tissue, human colon tissue, human lung cancer tissue, human pancreas cancer tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66868-1-Ig targets CD8a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11111

Product name: Recombinant human CD8A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-183 aa of BC025715

Sequence: QFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGCYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDI

相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2882205
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP01732
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

Protocols

Product Specific Protocols
IF protocol for CD8a antibody 66868-1-IgDownload protocol
IHC protocol for CD8a antibody 66868-1-IgDownload protocol
WB protocol for CD8a antibody 66868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Signal Transduct Target Ther

Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy

Authors - Xiao Hu
View Article
IF

Cancer Cell

Spatial determinants of antibody-drug conjugate SHR-A1811 efficacy in neoadjuvant treatment for HER2-positive breast cancer

Authors - Ding Ma
View Article
human

Cancer Cell

Proteogenomic characterization of non-functional pancreatic neuroendocrine tumors unravels clinically relevant subgroups

Authors - Shunrong Ji
View Article
IHC

Mol Cancer

The construction of modular universal chimeric antigen receptor T (MU-CAR-T) cells by covalent linkage of allogeneic T cells and various antibody fragments

Authors - Tao Chen
View Article
humanIHC

Mol Cancer

ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma.

Authors - Shiqi Ye
View Article

Cell Metab

Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1

Authors - Yizeng Fan
View Article
||
New chat

Able™

正在加载，请稍候...