Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse heart tissue, HeLa cells, LNCaP cells, HepG2 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85625-1-RR targets TACO1/CCDC44 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15688 Product name: Recombinant human CCDC44 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC007744 Sequence: MSAWAAASLSRAAARCLLARGPGVRAAPPRDPRPSHPEPRGCGAAPGRTLHFTAAVPAGHNKWSKVRHIKGPKDVERSRIFSKLCLNIRLAVKEGGPNPEHNSNLANILEVCRSKHMPKSTIETALKMEKSKDTYLLYEGRGPGGSSLLIEALSNSSHKCQADIRHILNKNGGVMAVGARHSFDKKGVIVVEVEDREKKAVNLERALEMAIEAGAEDVKETEDEEERNVFKFICDASSLHQVRKKLDSLGLCSVSCALEFIPNSKVQLAEPDLEQAAHLIQALSNHEDVIHVYDNIE 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 44 |
| Calculated molecular weight | 297 aa, 32 kDa |
| Observed molecular weight | 30-32 kDa |
| GenBank accession number | BC007744 |
| Gene Symbol | TACO1 |
| Gene ID (NCBI) | 51204 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BSH4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TACO1 (translational activator of cytochrome oxidase subunit I) is required for COXI translation. Mutations in TACO1 cause Leigh syndrome (LS) and cytochrome c oxidase deficiency. (27319982)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TACO1/CCDC44 antibody 85625-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |