Validation Data Gallery
Tested Applications
| Positive Dot Blot detected in | RNA |
Recommended dilution
| Application | Dilution |
|---|---|
| DOT BLOT | DOT BLOT : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68636-1-Ig targets 1-Methyladenosine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.
| Tested Reactivity | chemical compound |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PTG 相同性解析による交差性が予測される生物種 |
| Full Name | 1-Methyladenosine |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3085325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf