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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

14-3-3 Monoclonal antibody, PBS Only

14-3-3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10A3

Cat no : 66061-1-PBS

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using 14-3-3 Monoclonal antibody (66061-1-Ig)

    WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66061-1-PBS targets 14-3-3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 fusion protein Ag6055 相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 31 kDa, 28 kDa
GenBank accession numberBC056867
Gene symbol YWHAQ
Gene ID (NCBI) 10971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.