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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 14-3-3 Sigma Polyclonal antibody (10622-1-AP)

    WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using 14-3-3 Sigma Polyclonal antibody (10622-1-AP)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using 14-3-3 Sigma Polyclonal antibody (10622-1-AP)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using 14-3-3 Sigma Polyclonal antibody (10622-1-AP)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using 14-3-3 Sigma Polyclonal antibody (10622-1-AP)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

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14-3-3 Sigma Polyclonal antibody, PBS Only

14-3-3 Sigma Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10622-1-PBS targets 14-3-3 Sigma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag0973 相同性解析による交差性が予測される生物種
Full Name stratifin
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC002995
Gene Symbol 14-3-3 Sigma
Gene ID (NCBI) 2810
RRIDAB_2254479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31947
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.