• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

14-3-3 Sigma Monoclonal antibody, PBS Only

14-3-3 Sigma Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10D9

Cat no : 66251-1-PBS

Synonyms

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    WB analysis of HeLa using 66251-1-Ig (same clone as 66251-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    WB analysis of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    WB analysis of A431 using 66251-1-Ig (same clone as 66251-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human breast cancer using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human lung cancer using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using 14-3-3 Sigma Monoclonal antibody (66251-1-Ig)

    IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66251-1-PBS targets 14-3-3 Sigma in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag12644 相同性解析による交差性が予測される生物種
Full Name stratifin
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC002995
Gene symbol 14-3-3 Sigma
Gene ID (NCBI) 2810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.