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  • Featured Product
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14-3-3 Epsilon Polyclonal antibody

14-3-3 Epsilon Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11648-2-AP

Synonyms

14-3-3E, YWHAE, 14-3-3epsilon, 14-3-3 protein epsilon

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    WB analysis of A375 using 11648-2-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    WB analysis of HeLa using 11648-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A375 cells using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IP experiment of A375 using 11648-2-AP

    IP result of anti-14-3-3 Epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human colon using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human colon using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human lung cancer using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human lung cancer using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human lung cancer using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human lung cancer using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of mouse brain using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of mouse brain using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IHC staining of human gliomas using 11648-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11648-2-AP (14-3-3 epsilon antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using 14-3-3 Epsilon Polyclonal antibody (11648-2-AP)

    IF Staining of HepG2 using 11648-2-AP

    Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA375 cells, HeLa cells
Positive IP detected inA375 cells
Positive IHC detected inhuman colon tissue, human gliomas tissue, human lung cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11648-2-AP targets 14-3-3 Epsilon in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 Epsilon fusion protein Ag2247 相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC000179
Gene symbol 14-3-3E
Gene ID (NCBI) 7531
RRIDAB_2217787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IHC protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IF protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
IP protocol for 14-3-3 Epsilon antibody 11648-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Immunol

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Authors - Ying Kai Chan
View Article
humanWB

Nat Commun

Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation

Authors - Jiayu Liu
View Article
humanIP

Cell Host Microbe

Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.

Authors - William Riedl
View Article
mouseWB

EMBO J

AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

Authors - Xin Zheng
View Article
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
View Article
mouseWB

Neurobiol Dis

Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models

Authors - Rodrigo Vinueza-Gavilanes
View Article