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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

14-3-3E Monoclonal antibody, PBS Only

14-3-3E Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A3A4

Cat no : 66946-1-PBS

Synonyms

14 3 3 protein epsilon, 14 3 3E, 14-3-3E, FLJ45465, KCIP 1, MDCR, MDS, YWHAE

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of HeLa using 66946-1-Ig (same clone as 66946-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of HEK-293 using 66946-1-Ig (same clone as 66946-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of MCF-7 using 66946-1-Ig (same clone as 66946-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of Jurkat using 66946-1-Ig (same clone as 66946-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of HSC-T6 using 66946-1-Ig (same clone as 66946-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of NIH/3T3 using 66946-1-Ig (same clone as 66946-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using 14-3-3E Monoclonal antibody (66946-1-Ig)

    WB analysis of RAW 264.7 using 66946-1-Ig (same clone as 66946-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using 14-3-3E Monoclonal antibody (66946-1-Ig)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using 14-3-3E Monoclonal antibody (66946-1-Ig)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66946-1-PBS targets 14-3-3E in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen 14-3-3E fusion protein Ag25186 相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC000179
Gene symbol 14-3-3E
Gene ID (NCBI) 7531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.