Validation Data Gallery
Tested Applications
| Positive ELISA detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
28882-1-AP targets SARS-CoV-2 Membrane Glycoprotein in WB, IF, ELISA applications and shows reactivity with virus samples.
| Tested Reactivity | virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SARS-CoV-2 Membrane Glycoprotein fusion protein Ag30691 相同性解析による交差性が予測される生物種 |
| Full Name | COVID-19 M Protein |
| GenBank accession number | NC_045512 |
| Gene Symbol | SARS-CoV-2 Membrane Protein |
| Gene ID (NCBI) | 43740571 |
| RRID | AB_2881226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DTC5 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).
Publications
| Species | Application | Title |
|---|---|---|
J Virol Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation |