SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal antibody
SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Virus and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28769-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive ELISA detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
28769-1-AP targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.
| Tested Reactivity | Virus |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 相同性解析による交差性が予測される生物種 |
| Full Name | COVID-19 N Protein |
| GenBank accession number | NC_045512 |
| Gene symbol | COVID-19 N Protein |
| Gene ID (NCBI) | 43740575 |
| RRID | AB_2881212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 28769-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules. | ||
Antiviral Res RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region | ||
Virol J LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19 |
