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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6D10E2

Cat no : 67666-2-Ig

Synonyms

COVID-19 N protein, SARS-COV-2 N protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-2-Ig)

    WB analysis of Recombinant protein using 67666-2-Ig

    E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-2-Ig)

    ELISA experiment of Recombinant protein using 67666-2-Ig

    Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-2-Ig)

    ELISA experiment of Recombinant protein using 67666-2-Ig

    Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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Tested Applications

Positive WB detected inAg30676
Positive ELISA detected inRecombinant protein protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67666-2-Ig targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 相同性解析による交差性が予測される生物種
Full Name COVID-19 N Protein
GenBank accession numberNC_045512
Gene symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2882863
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 67666-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols