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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP3CC-specific Polyclonal antibody

PPP3CC-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55163-1-AP

Synonyms

PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of fetal human brain using 55163-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human testis using 55163-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    IHC staining of human stomach using 55163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    IF Staining of U2OS using 55163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, human testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55163-1-AP targets PPP3CC-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_005605
Gene symbol PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10860906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.

Protocols

Product Specific Protocols
WB protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
IHC protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
IF protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biomater

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Authors - Hong Cao
View Article
ratIHC

BMC Pregnancy Childbirth

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy

Authors - Yan Fang
View Article