ChromoTek 3xDYKDDDDK-peptide
HPLC-purified 3xDYKDDDDK-peptide
Cat no : fp
Synonyms
Validation Data Gallery
![DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.](https://www.ptglab.com/products/pictures/Result-fp.png "DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.")
Product Information
HPLC-purified 3xDYKDDDDK-peptide
| Description | 3xDYKDDDDK-peptide, also known as 3xFlag®-peptide |
| Applications | Mild elution of DYKDDDDK-tagged fusion proteins bound to DYKDDDDK Fab-Trap or anti-DYKDDDDK antibodies. |
| Sequence | MDYKDHDG DYKDHDI DYKDDDDK |
| Form | Lyophilized powder |
| Conjugate | Unconjugated |
| Molecular weight | 2,862 g/mol |
| Purity | >96% (HPLC) |
| Reconstitution | Dissolve 1 mg 3xDYKDDDDK-peptide in 0.5 mL PBS pH 7.4 to a final concentration of 2 mg/mL (0.7 mM). Vortex for a few minutes to dissolve the powder. Aliquot upon reconstitution. |
| Dilution | Dilute 3xDYKDDDDK-peptide stock to 150 µg/mL (0.05 mM) in PBS for elution of DYKDDDDK-tagged fusion proteins. |
| Storage Condition | Upon receipt store at -20°C/-4°F. Aliquot upon reconstitution and store at -20°C/-4°F. Avoid repeated freeze / thaw cycles. |
| Stability | Powder stable for 1 year upon receipt |
| Shipment | Shipped at ambient temperature |
Publications
| Application | Title |
|---|---|
Curr Biol Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria |