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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 4-1BBL/TNFSF9 Recombinant antibody (84185-5-RR)

    WB analysis using 84185-5-RR (same clone as 84185-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84185-5-RR (4-1BBL/TNFSF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84185-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using 4-1BBL/TNFSF9 Recombinant antibody (84185-5-RR)

    IF Staining of Raji using 84185-5-RR (same clone as 84185-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using 4-1BBL/TNFSF9 antibody (84185-5-RR, Clone: 241386E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84185-5-PBS in a different storage buffer formulation.

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4-1BBL/TNFSF9 Recombinant antibody, PBS Only

4-1BBL/TNFSF9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84185-5-PBS
Clone No.241386E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84185-5-PBS targets 4-1BBL/TNFSF9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 9
Calculated molecular weight 254 aa, 27 kDa
Observed molecular weight35 kDa
GenBank accession numberBC104805
Gene Symbol TNFSF9
Gene ID (NCBI) 8744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP41273
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.