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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 4EBP1 Recombinant antibody (83890-5-RR)

    WB analysis using 83890-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83890-5-RR (4EBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using 4EBP1 Recombinant antibody (83890-5-RR)

    FC experiment of HeLa using 83890-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HEK-293 cells using 4EBP1 Recombinant antibody (83890-5-RR)

    FC experiment of HEK-293 using 83890-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83890-5-RR

    Affinity and Kinetic Characterization of 83890-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83890-5-RR against Human 4EBP1 were performed. The affinity constant is 0.752 nM.

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4EBP1 Recombinant antibody

4EBP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83890-5-RR
Clone No.241055E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HL-60 cells, HepG2 cells, HeLa cells, K-562 cells, A431 cells
Positive FC (Intra) detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83890-5-RR targets 4EBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen 4EBP1 fusion protein Ag5056 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated molecular weight 118 aa, 12 kDa
Observed molecular weight15-20 kDa
GenBank accession numberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
RRIDAB_3671473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13541
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)

Protocols

Product Specific Protocols
WB protocol for 4EBP1 antibody 83890-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols