4EBP1 Recombinant antibody
4EBP1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241055E7
Cat no : 83890-5-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HL-60 cells, HepG2 cells, HeLa cells, K-562 cells, A431 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83890-5-RR is 4EBP1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | 4EBP1 fusion protein Ag5056 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 4E binding protein 1 |
Calculated molecular weight | 118 aa, 12 kDa |
Observed molecular weight | 15-20 kDa |
GenBank accession number | BC004459 |
Gene symbol | EIF4EBP1 |
Gene ID (NCBI) | 1978 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)
Protocols
Product Specific Protocols | |
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WB protocol for 4EBP1 antibody 83890-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |