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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 5 Lipoxygenase Polyclonal antibody (10021-1-Ig)

    WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using 5 Lipoxygenase Polyclonal antibody (10021-1-Ig)

    WB analysis of K-562 using 10021-1-Ig (same clone as 10021-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using 5 Lipoxygenase Polyclonal antibody (10021-1-Ig)

    WB analysis of HL-60 using 10021-1-Ig (same clone as 10021-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using 5 Lipoxygenase Polyclonal antibody (10021-1-Ig)

    WB analysis of DU 145 using 10021-1-Ig (same clone as 10021-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 5 Lipoxygenase Polyclonal antibody (10021-1-Ig)

    IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

    Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10021-1-PBS targets 5 Lipoxygenase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name arachidonate 5-lipoxygenase
Calculated molecular weight 674 aa, 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC130332
Gene Symbol 5 Lipoxygenase
Gene ID (NCBI) 240
RRIDAB_513237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09917
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).