Alpha 1B-Glycoprotein Polyclonal antibody
Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 14181-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue |
Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
14181-1-AP targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha 1B-Glycoprotein fusion protein Ag5414 相同性解析による交差性が予測される生物種 |
Full Name | alpha-1-B glycoprotein |
Calculated molecular weight | 54 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC035719 |
Gene symbol | A1BG |
Gene ID (NCBI) | 1 |
RRID | AB_10640441 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-AP | Download protocol |
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-AP | Download protocol |
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |