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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

A1CF Polyclonal antibody

A1CF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25812-1-AP

Synonyms

APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using A1CF Polyclonal antibody (25812-1-AP)

    WB analysis using 25812-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25812-1-AP (A1CF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Polyclonal antibody (25812-1-AP)

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Polyclonal antibody (25812-1-AP)

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25812-1-AP targets A1CF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A1CF fusion protein Ag22929 相同性解析による交差性が予測される生物種
Full Name APOBEC1 complementation factor
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC054873
Gene symbol A1CF
Gene ID (NCBI) 29974
RRIDAB_3669501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

A1CF, also known as Apobec-1 complementation factor, is an RNA-binding protein that plays a crucial role in the post-transcriptional editing of apolipoprotein B (apoB) mRNA. It is a component of the enzyme complex responsible for converting a CAA codon for glutamine to a UAA stop codon in apoB mRNA, resulting in the production of a truncated protein, apoB48, instead of the full-length apoB100.

Protocols

Product Specific Protocols
WB protocol for A1CF antibody 25812-1-APDownload protocol
IHC protocol for A1CF antibody 25812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols