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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

A1CF Recombinant antibody

A1CF Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241644F10

Cat no : 84322-4-RR

Synonyms

APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64, ACF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using A1CF Recombinant antibody (84322-4-RR)

    WB analysis using 84322-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84322-4-RR (A1CF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Recombinant antibody (84322-4-RR)

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using A1CF Recombinant antibody (84322-4-RR)

    IHC staining of mouse colon using 84322-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84322-4-RR (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84322-4-RR

    Affinity and Kinetic Characterization of 84322-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84322-4-RR against Human A1CF were performed. The affinity constant is 0.191 nM.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84322-4-RR is A1CF Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen A1CF fusion protein Ag22929 相同性解析による交差性が予測される生物種
Full Name APOBEC1 complementation factor
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC054873
Gene symbol A1CF
Gene ID (NCBI) 29974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for A1CF antibody 84322-4-RRDownload protocol
IHC protocol for A1CF antibody 84322-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols