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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha-2-Macroglobulin Polyclonal antibody

Alpha-2-Macroglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13545-1-AP

Synonyms

A2M, C3 and PZP like alpha 2 macroglobulin domain containing protein 5, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    WB analysis of human plasma using 13545-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    WB analysis of human brain using 13545-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    WB analysis of human brain using 13545-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    WB analysis of HepG2 using 13545-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    IP experiment of HepG2 using 13545-1-AP

    IP result of anti-Alpha-2-Macroglobulin (IP:13545-1-AP, 4ug; Detection:13545-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    IHC staining of human liver using 13545-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    IHC staining of human liver using 13545-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    IHC staining of human colon using 13545-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13545-1-AP (Alpha-2-Macroglobulin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-2-Macroglobulin Polyclonal antibody (13545-1-AP)

    IF Staining of HepG2 using 13545-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (13545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman blood tissue, human brain tissue, HepG2 cells, human plasma tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13545-1-AP targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha-2-Macroglobulin fusion protein Ag4489 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight 1474 aa, 163 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC040071
Gene symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_10642837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IHC protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
IP protocol for Alpha-2-Macroglobulin antibody 13545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer's Disease Patients.

Authors - Riikka Lampinen
View Article
humanIF

Am J Cancer Res

Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers

Authors - Mateusz Olbromski
View Article
Plasmodium falciparumWB

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan
View Article