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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

A4GNT Polyclonal antibody

A4GNT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22670-1-AP

Synonyms

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using A4GNT Polyclonal antibody (22670-1-AP)

    WB analysis using 22670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using A4GNT Polyclonal antibody (22670-1-AP)

    WB analysis of mouse pancreas using 22670-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    WB analysis of mouse stomach using 22670-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    WB analysis of rat stomach using 22670-1-AP

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of human stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of mouse stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of human pancreas cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of human pancreas cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of rat stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of rat stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using A4GNT Polyclonal antibody (22670-1-AP)

    IHC staining of mouse stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inK-562 cells, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue, L02 cells
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22670-1-AP targets A4GNT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A4GNT fusion protein Ag18432 相同性解析による交差性が予測される生物種
Full Name alpha-1,4-N-acetylglucosaminyltransferase
Calculated molecular weight 340 aa, 39 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC119639
Gene symbol A4GNT
Gene ID (NCBI) 51146
RRIDAB_11183768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

Protocols

Product Specific Protocols
WB protocol for A4GNT antibody 22670-1-APDownload protocol
IHC protocol for A4GNT antibody 22670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols