AADACL1 Polyclonal antibody

AADACL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14021-1-AP

Synonyms

EC:3.1.1.71, EC:3.1.1.-, Arylacetamide deacetylase-like 1, Acetylalkylglycerol acetylhydrolase, AADACL 1


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Tested Applications

Positive WB detected inPC-3 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14021-1-AP targets AADACL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AADACL1 fusion protein Ag5136 相同性解析による交差性が予測される生物種
Full Name arylacetamide deacetylase-like 1
Calculated molecular weight 46 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC047588
Gene symbol AADACL1
Gene ID (NCBI) 57552
RRIDAB_2034888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AADACL1 antibody 14021-1-APDownload protocol
IHC protocol for AADACL1 antibody 14021-1-APDownload protocol
IF protocol for AADACL1 antibody 14021-1-APDownload protocol
IP protocol for AADACL1 antibody 14021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP,IF

Cardiovasc Diabetol

Deficiency of neutral cholesterol ester hydrolase 1 (NCEH1) impairs endothelial function in diet-induced diabetic mice

Authors - Hai-Jian Sun
ratWB

J Proteomics

Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae.

Authors - Jingyi Duan
humanIHC

J Transl Med

Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer

Authors - Wei Tang
humanWB

Sci Rep

Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages

Authors - Ryosuke Shimai
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics

Authors - Junyi Zhang