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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AAMP Polyclonal antibody

AAMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21220-1-AP

Synonyms

Angio-associated migratory cell protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AAMP Polyclonal antibody (21220-1-AP)

    WB analysis using 21220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using AAMP Polyclonal antibody (21220-1-AP)

    WB analysis of mouse lung using 21220-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using AAMP Polyclonal antibody (21220-1-AP)

    WB analysis of human heart using 21220-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AAMP Polyclonal antibody (21220-1-AP)

    WB analysis of mouse kidney using 21220-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AAMP Polyclonal antibody (21220-1-AP)

    IHC staining of human liver cancer using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AAMP Polyclonal antibody (21220-1-AP)

    IHC staining of human liver cancer using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using AAMP Polyclonal antibody (21220-1-AP)

    IHC staining of human hepatocirrhosis using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using AAMP Polyclonal antibody (21220-1-AP)

    IHC staining of human hepatocirrhosis using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AAMP Polyclonal antibody (21220-1-AP)

    IF Staining of MCF-7 using 21220-1-AP

    Immunofluorescent analysis of MCF-7 cells using 21220-1-AP (AAMP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHUVEC cells, human heart tissue, mouse kidney tissue, mouse lung tissue, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21220-1-AP targets AAMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AAMP fusion protein Ag15655 相同性解析による交差性が予測される生物種
Full Name angio-associated, migratory cell protein
Calculated molecular weight 434 aa, 47 kDa
Observed molecular weight 52-58 kDa
GenBank accession numberBC039866
Gene symbol AAMP
Gene ID (NCBI) 14
RRIDAB_10733642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AAMP antibody 21220-1-APDownload protocol
IHC protocol for AAMP antibody 21220-1-APDownload protocol
IF protocol for AAMP antibody 21220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Exp Ther Med

MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB.

Authors - Xiaogao Wang
View Article