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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AARSD1 Polyclonal antibody (14900-1-AP)

    WB analysis using 14900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AARSD1 Polyclonal antibody (14900-1-AP)

    WB analysis of mouse brain using 14900-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using AARSD1 Polyclonal antibody (14900-1-AP)

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using AARSD1 Polyclonal antibody (14900-1-AP)

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AARSD1 Polyclonal antibody (14900-1-AP)

    IHC staining of mouse testis using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AARSD1 Polyclonal antibody (14900-1-AP)

    IHC staining of mouse testis using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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AARSD1 Polyclonal antibody

AARSD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14900-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AARSD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14900-1-AP targets AARSD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AARSD1 fusion protein Ag6692 相同性解析による交差性が予測される生物種
Full Name alanyl-tRNA synthetase domain containing 1
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC004172
Gene Symbol AARSD1
Gene ID (NCBI) 80755
RRIDAB_2257630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AARSD1(Alanyl-tRNA synthetase domain-containing protein 1) is also named as alanyl-tRNA editing protein Aarsd1 and belongs to the class-II aminoacyl-tRNA synthetase family. It plays a role in prevention of synthesis of proteins containing errors (mistranslation)(PMID:18172502). It has 3 isoforms produced by alternative splicing with the molecular weight of 45 kDa, 59 kDa and 66 kDa.

Protocols

Product Specific Protocols
WB protocol for AARSD1 antibody 14900-1-APDownload protocol
IHC protocol for AARSD1 antibody 14900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols