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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AASDHPPT Polyclonal antibody

AASDHPPT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11244-1-AP

Synonyms

CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AASDHPPT Polyclonal antibody (11244-1-AP)

    WB analysis of human brain using 11244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AASDHPPT Polyclonal antibody (11244-1-AP)

    IF Staining of HeLa using 11244-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11244-1-AP (AASDHPPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11244-1-AP targets AASDHPPT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AASDHPPT fusion protein Ag1763 相同性解析による交差性が予測される生物種
Full Name aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC016728
Gene symbol AASDHPPT
Gene ID (NCBI) 60496
RRIDAB_2304862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AASDHPPT antibody 11244-1-APDownload protocol
IF protocol for AASDHPPT antibody 11244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

bioRxiv

Mitochondrial Phosphopantetheinylation is Required for Oxidative Function

Authors - Pieter R Norden
View Article