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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCA7 Monoclonal antibody

ABCA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2E1A10

Cat no : 67128-1-Ig

Synonyms

ABCA SSN, ABCA7, ABCX, Autoantigen SS N, FLJ40025, Macrophage ABC transporter

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    WB analysis of rat brain using 67128-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ABCA7 Monoclonal antibody (67128-1-Ig)

    WB analysis of Jurkat using 67128-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig thymus tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    WB analysis of pig thymus using 67128-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    WB analysis of pig brain using 67128-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    WB analysis of mouse brain using 67128-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thymus tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    IHC staining of human thymus using 67128-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    IHC staining of human thymus using 67128-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ABCA7 Monoclonal antibody (67128-1-Ig)

    IF Staining of human tonsillitis using 67128-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat brain tissue, Jurkat cells, pig thymus tissue, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67128-1-Ig targets ABCA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ABCA7 fusion protein Ag27499 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family A (ABC1), member 7
Calculated molecular weight 234 kDa
Observed molecular weight 250 kDa
GenBank accession numberNM_019112
Gene symbol ABCA7
Gene ID (NCBI) 10347
RRIDAB_2882427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCA7 antibody 67128-1-IgDownload protocol
IHC protocol for ABCA7 antibody 67128-1-IgDownload protocol
IF protocol for ABCA7 antibody 67128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols